Translational_Unit
BMST1

Part:BBa_K1065100

Designed by: Caterina Marchioretti Michele Pedrotti   Group: iGEM13_UNITN-Trento   (2013-06-25)
Revision as of 11:31, 27 September 2013 by Cate (Talk | contribs)

BSMT1 enzyme

This BioBrick contains the coding sequence for BSMT1 (S-adenosylmethionine-dependent methyltransferase), it was derived from a Petunia hybrida gene and it catalyzes the reaction neeeded to product Methyl Salicylate from Salicylic Acid. This part was cloned by the iGEM Trento 2013 team for the creation of Wintergreen Device BBa_K1065106.

It was extrated from the iGEM Registry 2012 and


Usage and Biology

<img style="width:800px;"src="Tn-2013_ETH_detection.jpg">

Figure 2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 223


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Parameters
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