Device

Part:BBa_K1141002:Design

Designed by: Quentin LUBART   Group: iGEM13_Grenoble-EMSE-LSU   (2013-09-27)
Revision as of 11:17, 27 September 2013 by Registry (Talk | contribs)

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Plac-RBS-KillerRed (IPTG-inducible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 289
    Illegal BsaI.rc site found at 580


Design Notes

There was an Ecor1 restriction site. We removed this site in order to made this biobrick compatible to the 3A assembly.


Source

KillerRed came from addgene plasmid pBabe-KR-CENPA

References