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Coding
TALEA-dife

Part:BBa_K1189021

Designed by: Chris Wintersinger, Denny Hoang, Taylor Remondini   Group: iGEM13_Calgary   (2013-09-17)
Revision as of 08:48, 27 September 2013 by Dwoppers (Talk | contribs)

TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

This part was created by the fusion of the heavy and light ferritin subunits (PARTSTS) to form the form the fusion subunit. The lacI promoter (PART), double terminator (PART) and his tag was also added in order to induce protein expression and allow purification. This part is similar to ____ PART, but instead of the E-coil, it is replaced by TALB(PART). This part was primarily used as a backup in the case our coiled-coil systems did not bind to eachother. It was intended to have the same functions as _____ PART___, but with the additional DNA binding capabilities given by TALB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3656


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Parameters
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