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Part:BBa_K1228006

Designed by: Zhang SW   Group: iGEM13_HZAU-China   (2013-09-09)
Revision as of 07:42, 27 September 2013 by Zhevol (Talk | contribs) (→‎Reference)

25aa with T1 terminator and Constitutive promoter veg

Lf (loctoferrin) is located in many key body fluids , e.g., milk, tears, saliva and other secretions, and in white blood cells ,it has become clear that it is an important part of mammalian defence mechanisms, with a wide range of protective functions.25 amino acid residues is from Lf but has a strong bactericidal, bacteriostatic ability, and the antimicrobial activity than natural LF.We add T1 terminator and Constitutive promoter veg on the 25aa to make it can work regularly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. Biochimicaet BiophysicaActa,1992,1121( 1/2):130-136. EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovine lactoferricin[J]． Anticancer Research,2002,22(5):2703－2710. Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.

[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
n/a25aa with T1 terminator and Constitutive promoter veg