Part:BBa_K1189018

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.

Figure 4. Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.

The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
<p>Figure 11. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.

Something about scaffold…

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1289