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Composite
gfp+luxI

Part:BBa_K1036002:Design

Designed by: Xin Guo, Xi Xi   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 06:40, 27 September 2013 by Tina Zhang (Talk | contribs) (→‎Design Notes)


gfp (with LVA-tag) under plux R control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662
    Illegal BsaI.rc site found at 920
    Illegal BsaI.rc site found at 2023


Design Notes

This part contains two subparts: BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D). BBa_I763020 digested by EcoR & SpeI and BBa_K546000 digested by EcoR & XbaI were ligated together by T4 ligase.

Source

iGEM

References

Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)