Part:BBa_K1139100

Adenylate isopentenyltransferase 4

In order to construct cytokinin production part, we focused on AtIPT, a plant enzyme which catalyzes the synthesis of cytokinin. The mechanisms of cytokinin synthesis are shown in Fig. 1 (Takei et al., 2001). We ordered a DNA sequence of AtIPT (AtIPT4) derived from A. thaliana and are now constructing the part including one of them.

Fig. 1. Mechanisms of cytokinin synthesis

At<i>IPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in <i>E. coli. It is also expected in the report that E. coli also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP.

For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Project/Farming our work in Tokyo_Tech 2013 wiki].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 328
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 61
  
• 1000
  COMPATIBLE WITH RFC[1000]