Composite

Part:BBa_K1036003:Design

Designed by: Xin Guo, Xi Xi   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 06:36, 27 September 2013 by Tina Zhang (Talk | contribs) (→‎Design Notes)

lux pL controlled luxR with lux pR controlled gfp (LVA-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3909
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828
    Illegal BsaI.rc site found at 2086
    Illegal BsaI.rc site found at 3189


Design Notes

This part contains two subparts: BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D).BBa_I763020 digested by EcoR & SpeI and BBa_K546000 digested by EcoR & XbaI were ligated together by T4 ligase.

Source

iGEM

References

Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)