Composite

Part:BBa_K1074007

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-11)
Revision as of 19:04, 26 September 2013 by Nocturne (Talk | contribs)

Pctc+RBS+amilCP

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress. amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. We assemble this gene circuit to expect a visual signal when B.subtilis grow to the post-log phase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 78
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 78
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 78
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None