Coding
ndh-lva

Part:BBa_K1036001:Design

Designed by: Shengquan Zeng, Zhaopeng Cheng   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 16:59, 26 September 2013 by Tina Zhang (Talk | contribs) (→‎Source)


the ndh gene coding for respiratory NADH dehydrogenase II


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the LVA-tag was added


Source

The ndh gene was amplifed from the genome DNA of E.coli strain BL21 via PCR.

References

Messner, K. R. & Imlay, J. A. The identification of primary sites of superoxide and hydrogen peroxide formation in the aerobic respiratory chain and sulfite reductase complex of Escherichia coli. J. Biol. Chem. 274, 10119-10128 (1999)