Composite
M13 genes

Part:BBa_K1139019:Design

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-01)
Revision as of 15:53, 26 September 2013 by Fujimon (Talk | contribs)

Promoterless-M13 on pSB3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6027
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick is derived from M13mp18 phage vector, amplified by using PCR.


Source

TBD

Fwd primer:5'-ACGTGAATTCGCGGCCGCTTCTAGAGAACCGGGGTACATATGATTGAC-3'

Rev primer:5'-CTGCAGCGGCCGCTACTAGTATTTCACCAGCGAGACGG-3'


References