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luxR-ndh

Part:BBa_K1036000:Design

Designed by: Shengquan Zeng, Zhaopeng Cheng   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 15:49, 26 September 2013 by Tina Zhang (Talk | contribs) (→‎Source)


lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101


Design Notes

The ndh gene was amplifed from the genome of E. coli strain BL21(DE3) via PCR. And we know that the biobrick BBa_F2621 (position in plate: 2012-Plate 2-21F) is a quorum sensing promoter. We digested ndh with EcoRI and Xba I, and 21F with EcoRI and SpeI. Then we constructed ndh plasmid by ligation.

Source

The ndh gene was amplifed from the genome DNA of E. coli strain BL21(DE3) via PCR.

References

1. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)