Coding

Part:BBa_K1151000

Designed by: Emanuele Rizzo   Group: iGEM13_UniSalento_Lecce   (2013-09-21)
Revision as of 12:19, 26 September 2013 by LeleBiotec (Talk | contribs) (→‎Usage and Biology)

Nickel-responsive pleiotropic regulator (HpNikR)

The HpNikR protein is a pleiotropic regulator from Helicobacter pylori. In presence of nickel it can acts as an activator or a repressor depending of the specific promoter that contains its operator site. It consists of two dimeric DNA binding domains separated by a tetrameric regulatory domain that binds nickel. This domain corresponds to the C-terminal regulatory domain which contains four nickel binding sites at the tetramer interface. Binding nickel, then a conformational change allows it to activate or repress trascription.


Usage and Biology

                                               Cattura.jpg                                           

                               Figure 1: HpNikR nickel-binding domain (tetramer).



Cattura1.jpg

Figure 2: Proteic sequence analysis with Uniprot.

Safety

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aNickel-responsive pleiotropic regulator (HpNikR)