Translational_Unit
AtIPT4

Part:BBa_K1139100

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-08-29)
Revision as of 11:46, 26 September 2013 by Keso57 (Talk | contribs)

Adenylate isopentenyltransferase 4

In order to construct cytokinin production part, we focused on AtIPT, a plant enzyme which catalyzes the synthesis of cytokinin. The mechanisms of cytokinin synthesis are shown in Fig. 1 (Takei et al., 2001). We ordered a DNA sequence of AtIPT (AtIPT4) derived from A. thaliana and are now constructing the part including one of them.

Fig. 1. Mechanisms of cytokinin synthesis

AtIPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in E. coli. It is also expected in the report that E. coli</> also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None