Part:BBa_K1065311
Blue-light photoinducible AmilCP + EFE producing device
This device is composed by the blue light sensor device, an inverter cassette , the reporter AmilCP and EFE (Ethylene Forming Enzyme) coding sequence. This device allows to produce AmilCP (a blue chromoprotein) and 2-Oxoglutarate Oxygenase/Decarboxylase enzyme when culture is exposed to blue light. To allow this behaviour an inverter cassette was included into the device. The cassette is compose by the cI coding sequence and the repressible promoter pLambda. Everything is under the control of promoter J23100.
This part was successfully cloned by UNITN-Trento 2013 iGEM team in order to design an ethylene producing device that is induced by blue light to speed up fruit ripening.
Parts from 2011 Uppsala-Sweden team and 2006 Berkeley team were used along with our new ethylene producing part.
SAFETY NOTES: this part is an ethylene producing system: ethylene is explosive at high concentration.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4114
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465
Illegal AgeI site found at 4865 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218
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