Device

Part:BBa_K1088011

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-28)
Revision as of 08:35, 26 September 2013 by P.R.A (Talk | contribs)

B. subtilis dxs (lac promoter without lac inhibitor)

This part has the dxs gene derived from B. subtilis (BBa_K1088000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway.

Fluorescence activated cell sorting (FACS) was used to examine the expression profile of a similar part (BBa_K1088008) with linker-GFP attached to the end of Dxs. The part was examined in the K-12 MG1655 (natural levels of LacI) and KG22 (overexpression of LacI from chromosome) strains. The experiment proved that overexpression of LacI is required for repression of the lac promoter (and therefore also control of the regulation). BBa_K1088027 is a similar part which overexpresses LacI from the part it self.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1279
    Illegal EcoRI site found at 1936
    Illegal PstI site found at 1337
    Illegal PstI site found at 1783
    Illegal NgoMIV site found at 1236
    Illegal AgeI site found at 1129
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1078
    Illegal SapI.rc site found at 1777


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