Coding

Part:BBa_K1151000:Design

Designed by: Emanuele Rizzo   Group: iGEM13_UniSalento_Lecce   (2013-09-21)
Revision as of 07:38, 26 September 2013 by LeleBiotec (Talk | contribs) (→‎References)


Nickel-responsive pleiotropic regulator (HpNikR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was inserted in a pET-15b plasmid vector, in order to have a better storage.


Source

It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.

References

1. Muller et al, Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori. Nucleic Acids Res. 2011; 39(17):7564-75. 2. Dian et al, Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel -bound States. J Mol Biol. 2006; 25;361(4):715-30. 3. Dosanjh et Michel, Microbial nickel metalloregulation: NikRs for nickel ions. Curr Opin Chem Biol. 2006 (2):123-30. 4. Ernst et al, The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori . Infect Immun. 2005; 73(11):7252-8.