Coding

Part:BBa_K1014000:Design

Designed by: Xiangmiao Zeng   Group: iGEM13_HIT-Harbin   (2013-08-28)
Revision as of 05:29, 26 September 2013 by Sema1688 (Talk | contribs) (→‎Design Notes)

HrpS Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 163
    Illegal AgeI site found at 298
    Illegal AgeI site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TAATAA double stop codon added. Codon optimization was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research). Results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]

Source

E.coli

References

Grote A， Hiller K， Scheer M et.al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host[J]. Nucleicacids Research, JUL 1 2005