Composite

Part:BBa_K1166002

Designed by: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero   Group: iGEM13_TecMonterrey   (2013-09-17)
Revision as of 21:39, 25 September 2013 by LuisFernandoCG (Talk | contribs)

HlyA-tag+Secretion system

This is a device that allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli. It’s designed so that the only thing that you have to do is to assemble your protein part in the device via the biofusion standard (BBF RFC 23), (Cut the device with EcoRI and XbaI, cut your protein part with EcoRI and SpeI, mix & ligate). This procedure will leave your part in frame with a signal peptide.

Note: It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C-terminus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1324
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1306


[edit]
Categories
Parameters
None