Composite
Part:BBa_K1140006
Designed by: Janssel Reyes del Castillo Group: iGEM13_UANL_Mty-Mexico (2013-09-13)
pTet + 37 oC RNA thermometer + mCherry (LVA)
mCherry coding sequence is under the regulation of a pTet promoter. Translation is also regulated by a synthetic RNA thermometer[1] that allows translation only over 37 °C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 676
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//function/reporter/fluorescence
//direction/forward
//function/reporter/fluorescence
direction | Forward |
function | Integrated Transcriptional and Translational regulation |
protein | mCherry |
switch_point | 37 celsius degrees |
tag | LVA |