Reporter
Part:BBa_K1031803
Designed by: He Shuaixin Group: iGEM13_Peking (2013-09-09)
Pu-B0031-sfGFP-Terminator (XylR)
Structure
Fig 1
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 167
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 219
Construction and tunning
Pc promoter J23114 is selected to initiate the transcription of XylR. Based on this circuit, we constructed a library of RBS (Ribosome Binding Site) including B0031[1], B0032[2], B0033[3] and B0034[4] for tunning for expression level of reporter gene sfGFP (Fig 2). K1031803 consists of Pu promoter, RBS B0031 and reporter gene sfGFP
Fig 2 Construction of reporter circuit. The orange arrow represents Pu promoter for XylR. The green oval stands for RBS B0031. sfGFP coding sequence is shown with dark blue, while terminator B0015[5] is in dark red.
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//rbs/prokaryote/constitutive
//terminator/double
//function/reporter/fluorescence
//rbs/prokaryote/constitutive
//terminator/double
device_type |