Reporter

Part:BBa_K1031803

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-09-09)
Revision as of 15:25, 25 September 2013 by Psyche (Talk | contribs)

Pu-B0031-sfGFP-Terminator (XylR)


Structure

Fig 1


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 219


Construction and tunning

Pc promoter J23114 is selected to initiate the transcription of XylR. Based on this circuit, we constructed a library of RBS (Ribosome Binding Site) including B0031[1], B0032[2], B0033[3] and B0034[4] for tunning for expression level of reporter gene sfGFP (Fig 2). K1031803 consists of Pu promoter, RBS B0031 and reporter gene sfGFP

Fig 2 Construction of reporter circuit. The orange arrow represents Pu promoter for XylR. The green oval stands for RBS B0031. sfGFP coding sequence is shown with dark blue, while terminator B0015[5] is in dark red.



[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
//rbs/prokaryote/constitutive
//terminator/double
Parameters
device_type