Signalling

Part:BBa_F2620

Designed by: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]   Group: iGEM04_MIT   (2004-08-09)
Revision as of 18:19, 18 July 2006 by Bcanton (Talk | contribs)

3OC6HSL -> PoPS Receiver

A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.

Usage and Biology

Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., Part:pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004

Device Characteristics

controlR0040
device_typeReceiver
family-NA-
latencySeconds
proteinsLuxR, TetR
signalling_molecule3OC6HSL
switch_point2nM

Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]

Transfer Function

Latency

Specificity

[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]

Stability

[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]

[edit]
Categories
//classic/signalling/receiver
//function/cellsignalling
Parameters
controlR0040
device_typeReceiver
family
latencySeconds
proteinsLuxR, TetR
signalling_molecule3OC6HSL
switch_point2nM