Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Project
c-di-AMP

Part:BBa_K1045002:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-06-24)
Revision as of 13:00, 25 September 2013 by Gundl (Talk | contribs) (→‎Design Notes)

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone (see BBa_K1045004). It was then further SpeI and EcoRI digested and ligated with the XbaI and EcoRI digested CFP (BBa-E0020). Altogether this composes BBa_K1045002.

Source

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965