Composite

Part:BBa_I13540

Designed by: Josh Michener   Group: iGEM04_MIT   (2005-08-22)
Revision as of 02:35, 18 July 2006 by Kchang17 (Talk | contribs)

Intermediate in two promoter screening plasmid

Can also be used as a GFP only control, to compare to measurements such as in pSB1A10. Pbad must be induced to high transcription, so I13540 should be used in strain CW2553/pJat8 with high arabinose induction such as 3e-3% (Horazdovsky BF and Hogg RW. Genetic reconstitution of the high-affinity L-arabinose transport system. J Bacteriol 1989 Jun; 171(6) 3053-9. pmid:2656640.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880
    Illegal SapI site found at 961


[edit]
Categories
//classic/composite/uncategorized
Parameters
None