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Composite

Part:BBa_K1119008

Designed by: Hyun Jung Lee   Group: iGEM13_Hong_Kong_HKUST   (2013-09-16)
Revision as of 09:02, 25 September 2013 by Hjleeab (Talk | contribs)

CMV promoter - GFP - hGH polyA tail

Composite part for characterization of CMV promoter. CMV (Cytomegalovirus) promoter is a constitutive mammalian promoter that we used to express glyoxylate genes in mammalian cell. Our objective in characterizing this promoter is to test if the CMV promoter drives the expression of green fluorescence protein (GFP) in mammalian HEK293FT cell line.

CMV promoter characterization construct composes of CMV promoter, Green Fluorescence Protein (GFP) and hGH polyadenylation sequence (hGH pA) in pSB1C3 backbone.(BBa_K1119006 – BBa_K648013 – BBa_K404108 – pSB1C3)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 628
    Illegal AgeI site found at 1369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1772
    Illegal BsaI.rc site found at 1274


[edit]
Categories
//chassis/eukaryote/human
Parameters
None