Coding
cheY

Part:BBa_C0020:Design

Designed by: Victoria Chou, Kenneth Nesmith, Madeleine Sheldon-Dante   Group: Antiquity   (2004-08-02)
Revision as of 17:10, 17 July 2006 by Smelissali (Talk | contribs) (→‎References: fixed link)


CheY "tumbling" chemotaxis coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changes from the NCBI sequence: A BioBricks restriction site for PstI was at position 260. As a result, the codon starting at position 262 was changed from "gca" to "gcg".

The terminating codon at the end, "tga", was changed to "taataa" as per BioBrick regulations as told to us by Randy Rettberg.


Source

NCBI - Escherichia coli K12 MG1655 (NC_000913)

References

  • NCBI sequence data[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=946393 available here]