Plasmid

Part:BBa_K1114409

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)
Revision as of 04:51, 25 September 2013 by THaddock (Talk | contribs)

DVL0_DG This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.

LacZ in pSB1C3 with DG fusion sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 245
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 245
    Illegal BamHI site found at 266
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8
    Illegal BsaI.rc site found at 522


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