RBS
Part:BBa_K1114111
Designed by: Traci Haddock Group: iGEM13_BostonU (2013-09-07)
Revision as of 03:45, 25 September 2013 by THaddock (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K1114111 short</partinfo> <html> This part is a <a href="href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> adapted bicistronic design (BCD) e...")
Bicistronic design RBS 16, MoClo Format with BC fusion sites
This part is a MoClo adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from Mutalik et al., 2013. It contains two Shine-Dalgarno sequences instead of the standard one in ribosomal binding units such as BBa_B0034. From the library of BCDs, this is a MoClo version of BCD16 (BioFAB # apFAB694) and we obtained the sequence data for this part from the BioFAB.
This BCD1 is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
This is Figure 1A from Mutalik et al., 2013 showing the basic structure of their BCD design. The BCD with its two Shine-Dalgarno motifs (SD1 and SD2) is shown in gray.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
| None |