Protein_Domain
flyPCD

Part:BBa_J176001

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-07-02)
Revision as of 03:11, 25 September 2013 by Kahaynes (Talk | contribs)

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flyPCD

Polycomb chromodomain from the Drosophila (fruit fly) Pc protein (a.a. 16-78).
Alias: KAH03


Usage and Biology

Drosophila PCD of Pc1 (orange) Crystal structure reported by Fischle et al. 2011. Grey = H3 histone tail; Ball and stick = trimethylated lysine 27
  • Protein Data Bank ID 1PDQ
  • Mammalian expression vector required
  • Protein domain; requires promoter, start codon, stop codon, and polyA signal for proper expression

See the human variant BBa_J176000 for more details on the biology of the Polycomb chromodomain.




REFERENCES:

  1. Fischle, W, Wang, Y, Jacobs, SA, Kim, Y, Allis, CD, Khorasanizadeh, S. (2003) Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains. Genes Dev. 17:1870-1881.
  2. Min, J, Zhang, Y, Xu, RM. (2003) Structural basis for the specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27. Genes Dev. 17:1823-1828.
  3. Haynes, KA, Silver, PA. (2011) Synthetic reversal of epigenetic silencing. J Biol Chem. 286:27176-27182.









Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/chromatinremodeling
//chassis/eukaryote/human
//proteindomain/binding
Parameters
chassismammalian cells
uniprotP26017