RBS

Part:BBa_K1114106

Designed by: Traci Haddock   Group: iGEM13_BostonU   (2013-09-07)
Revision as of 18:18, 24 September 2013 by THaddock (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K1114106 short</partinfo> <html> This part is a <a href="href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> adapted bicistronic design (BCD) e...")

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Bicistronic Design RBS 1, MoClo Format with BC fusion sites

This part is a MoClo adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from Mutalik et al., 2013. From the library of BCDs, this is a MoClo version of BCD1 (BioFAB # apFAB681) and we obtained the sequence data for this part from the BioFAB.

This BCD1 is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 694
    Illegal AgeI site found at 806
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None