Composite

Part:BBa_K1073029

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 18:01, 24 September 2013 by Kerstin K (Talk | contribs)

Inducible ampicillin resistance cassette + LuxR expression cassette

In complex with the autoinducer molecule N-3-oxo-hexanoyl-HSL, the transcription regulator LuxR binds to specific sequences in the promoter region and enables transcription of ampR. This construct provides all the parts required to express the gene downstream of the inducible promoter. Only LuxI, the synthase producing the autoinducer molecule N-3-oxo-hexanoyl-HSL is missing in the construct. However, N-3-oxo-hexanoyl-HSL can be taken up from the media.

Usage and Biology

The expression rate of the genes downstream of the promoter region can be tuned by the concentration of N-3-oxo-hexanoyl-HSL in the media. In this case the expression of beta lactamase can be induced by adding N-3-oxo-hexanoyl-HSL to the culture broth.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1098
    Illegal NheI site found at 1121
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None