Coding

Part:BBa_K1150002

Designed by: Freiburg 2013   Group: iGEM13_Freiburg   (2013-09-16)
Revision as of 17:35, 24 September 2013 by NightworkMax (Talk | contribs)

KRAB

KRAB
Function Repressor domain
Use in Mammalian cells, higher vertebrate organisms
RFC standard RFC 25
Backbone pSB1C3
Organism Homo sapiens
Source Martin Fussenegger, pSAM200
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB domains were attached to several DNA binding proteins such as tetR, TAL effectors and Cas9 (http://2013.igem.org/Team:Freiburg/Project/effector#repression) - thereby efficiently silencing gene expression downstream of desired target promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 244
    Illegal SapI.rc site found at 208


[edit]
Categories
Parameters
None