Reporter

Part:BBa_K1114500

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-12)
Revision as of 17:33, 24 September 2013 by THaddock (Talk | contribs)

MoClo Level 1 RFP reporter with AE fusion sites

pJ04B1Rm_AE

This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD1_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

This part contains a bicistronic design (BCD) element as the 5' UTR regulatory part from Mutalik et al., 2013. From the library of BCDs from Mutalik, this contains BCD2 and we obtained the sequence data for this part from the BioFAB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 694
    Illegal AgeI site found at 806
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None