Composite

Part:BBa_K1065305:Design

Designed by: Bruno Aor   Group: iGEM13_UNITN-Trento   (2013-09-09)
Revision as of 17:16, 24 September 2013 by Fabius924 (Talk | contribs) (→‎Design Notes)


Blue light sensor without inverter for the production of amilGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 634
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 1547
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 276
    Illegal AgeI site found at 1422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1311
    Illegal BsaI.rc site found at 175


Design Notes

Part BBa_K952003 was missing an RBS before the amilGFP CDS, thus it doesn't work. We successfullt inserted the RBS by mutagenesis

Source

Igem registry Kit 2013

References