Composite

Part:BBa_K1139021

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-09)
Revision as of 14:25, 24 September 2013 by Tryalnigro (Talk | contribs)

Plux-M13-Plac-GFP on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is extracted from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is altered to lux promoter. A phage particle is formed only when the host cell receives AHL signal(3OC6HSL, C6) because g2p(gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle.

As a reporter, GFP is inserted on the downstream of the lac promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 163
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7353


[edit]
Categories
Parameters
None