Coding
zif23
Part:BBa_C2000
Designed by: Ronny Krashinsky Group: Antiquity (2004-01-25)
Revision as of 23:53, 15 July 2006 by Smelissali (Talk | contribs)
Repressor, Zif23-GCN4 (RBS-, LVA+)
Coding region for Zif23-GCN4. This protein is constructed out of fingers 2 and 3 from Zif268 fused with the leucine zipper domain from GCN4. The protein binds cooperatively as a dimer to a symmetric target DNA sequence in which each monomer targets 5'-GCGTGG-3'.
variant available as Part:BBa_C2001
Usage and Biology
Wolfe et al., "Structure of a Designed Dimeric Zinc Finger Protein Bound to DNA" [http://pubs.acs.org/cgi-bin/sample.cgi/bichaw/2003/42/i46/html/bi034830b.html link] Biochemistry 42:13401
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
None |