Coding
zif23

Part:BBa_C2000

Designed by: Ronny Krashinsky   Group: Antiquity   (2004-01-25)
Revision as of 23:53, 15 July 2006 by Smelissali (Talk | contribs)

Repressor, Zif23-GCN4 (RBS-, LVA+)

Coding region for Zif23-GCN4. This protein is constructed out of fingers 2 and 3 from Zif268 fused with the leucine zipper domain from GCN4. The protein binds cooperatively as a dimer to a symmetric target DNA sequence in which each monomer targets 5'-GCGTGG-3'.

variant available as Part:BBa_C2001

Usage and Biology

Wolfe et al., "Structure of a Designed Dimeric Zinc Finger Protein Bound to DNA" [http://pubs.acs.org/cgi-bin/sample.cgi/bichaw/2003/42/i46/html/bi034830b.html link] Biochemistry 42:13401


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
None