Composite

Part:BBa_K1139021:Design

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-09-09)
Revision as of 12:44, 24 September 2013 by Shinya0825 (Talk | contribs) (→‎Source)


Plux-M13-Plac-GFP on pSB3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 163
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7353


Design Notes

TBD


Source

Lux promoter from oligo DNA(BBa_R0062), M13 genes and origin from BBa_K1139018, GFP from BBa_E0040, rbs from BBa_B0030, double terminator from BBa_B0015 are combined.

References