Generator

Part:BBa_K1060012

Designed by: Ingmar Claes   Group: iGEM13_KU_Leuven   (2013-09-24)
Revision as of 08:31, 24 September 2013 by Registry (Talk | contribs)

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Methyl Salicylate Production under TetR regulation

KU_Leuven 2013 requested the BBa_J45700 brick from MIT 2006 and recloned into the pSB1C3 standard plasmid backbone (BBa_K1060003). In this part we removed the lac promoter in front of the pchBA gene to get a constitutive expression of all genes needed for methyl salicylate production. We did this by amplifying the For the assembly of this part we refer to the original MIT brick BBa_J45700 => "We assembled a complete wintergreen odor biosynthetic system (BBa_J45700) by combining the salicylic acid (BBa_J45320) and wintergreen odor generators (BBa_J45120). BBa_J45700 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor."


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 746
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1656
    Illegal NgoMIV site found at 2060
    Illegal NgoMIV site found at 2185
    Illegal NgoMIV site found at 2196
    Illegal NgoMIV site found at 2477
    Illegal NgoMIV site found at 2945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287


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