Regulatory
pBad/araC

Part:BBa_I0500:Design

Designed by: Sri Kosuri   Group: Antiquity   (2003-12-05)
Revision as of 19:14, 14 July 2006 by Macowell (Talk | contribs) (→‎References: added Johnson and Schlief article)

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Inducible pBad/araC promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Source

Keasling Lab at UC Berkeley

References

  • Lee et al., "[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6262769 Mechanism of araC autoregulation and the domains of two overlapping promoters, PC and PBAD, in the L-arabinose regulatory region of Escherichia coli]", PNAS, Vol. 78, Num. 2, 752-756.
  • Johnson and Schleif, "[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7768852 In vivo induction kinetics of the arabinose promoters in Escherichia coli]", J Bacteriol, vol 177(12):3438-3422