Generator

Part:BBa_K1073014

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 20:00, 23 September 2013 by Laura S (Talk | contribs) (→‎Usage and Biology)

Constitutively expressed transcription regulator RhlR + LVA

This device consists of a strong constitutive promoter (BBa_J23100) combined with RBS (BBa_B0032) and the coding region of the transcription regulator RhlR (BBa_C0071). This results in a constitutively high transcription level of RhlR.


Usage and Biology

In combination with N-buturyl homoserine lactone (N-buturyl-HSL) synthesized by RhlI, RhlR is able to activate the inducible promoter of the rhl quorum sensing System of Pseudomonas aeruginosa.

RhlR binds to specific sequences in the promoter region and in the presence of N-buturyl-HSL it activates gene expression [Medina et al., 2003].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 777


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