![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1031911
Designed by: He Shuaixin Group: iGEM13_Peking (2013-09-01)
XylS-Terminator
XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding containing two helix-turn-helix motifs and an N-terminal domain required for effector binding and protein dimerization.
XylS detects benzoate and its’ derivatives, mainly methyl and chlorine substitutes at 2-, 3- carbon.
![](https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_Xyls_figure1.png)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 32
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 754
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 217
[edit]
Categories
Parameters
//cds/transcriptionalregulator/activator
//chassis/prokaryote
//chassis/prokaryote
biology | Pseudomonas putida |
protein | XylS |