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Experience: Works
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Project
DarR

Part:BBa_K1045017

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 13:52, 23 September 2013 by Nina Heckmann (Talk | contribs)

DarR reporter system

This composite part encodes a reporter system to monitor the repression mediated by DarR. DarR is a transcriptional repressor that binds to the DarR operator sequence blocking transcription of the downstream gene. These effects are enhanced by c-di-AMP. In part BBa_K1045017, the DarR operator is placed in between a strong promoter BBa_J23110 and a GFP generator BBa_E0240. DarR itself is part of an inverted expression unit with a weak promoter BBa_J23117. Transcription of the DarR mRNA might be terminated by an inverse terminator BBa_K104509. This system was designed to screen for compounds similar to c-di-AMP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 806
    Illegal NheI site found at 829
    Illegal NheI site found at 909
    Illegal NheI site found at 932
    Illegal NotI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 860
    Illegal AgeI site found at 168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1630


[edit]
Categories
Parameters
n/aDarR reporter system