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Part:BBa_K1045016:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 13:50, 23 September 2013 by Nina Heckmann (Talk | contribs)

Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 168
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The inverse DarR sequence was cut out from part BBa_K1045001 and ligated into part BBa_K1045009 in a post-fixing composition.


Source

To be continued

References