Composite
Part:BBa_K1045016:Design
Designed by: iGEM Team Göttingen 2013 Group: iGEM13_Goettingen (2013-09-20)
Revision as of 13:50, 23 September 2013 by Nina Heckmann (Talk | contribs)
Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 718
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 731
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 168
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The inverse DarR sequence was cut out from part BBa_K1045001 and ligated into part BBa_K1045009 in a post-fixing composition.
Source
To be continued