Part:BBa_K1045011

Promoter reverse

Part BBa_K1045011 encodes for a weak promoter based on BBa_J23117 promoter from the well characterized Anderson promoter collection. Yet, the promoter in BBa_K1045011 is inverted, i.e. the prefix is located at position -1. Additionally, BBa_K1045011 harbors extra bases in front of the promoter sequence. These extra bases might allow the cloning of a vector from which two genes can be expressed into diverging direction: You could place BBa_K1045011 in front of other promoters oriented in the usual way. The extra bases were added to prevent that the binding of the RNA polymerase to the usual promoter influences the binding of the RNA polymerase to the inverse promoter in BBa_K1045011. (see scheme, for an example see part BBa_K1045017, a screening system for compounds similar to c-di-AMP).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1
  Illegal NheI site found at 24
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 55
  
• 1000
  COMPATIBLE WITH RFC[1000]