Coding
"D"

Part:BBa_K1080002:Design

Designed by: Macquarie University   Group: iGEM13_Macquarie_Australia   (2013-09-22)
Revision as of 01:41, 23 September 2013 by DMC (Talk | contribs) (→‎Design Notes)


ChlD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2039
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 614
    Illegal NgoMIV site found at 1433
    Illegal NgoMIV site found at 1975
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence

Source

Chlamydomonas reinhardtii

References