Coding
"H"

Part:BBa_K1080001

Designed by: Macquarie University   Group: iGEM13_Macquarie_Australia   (2013-09-22)
Revision as of 01:25, 23 September 2013 by Kirsten (Talk | contribs)

ChlH


Magnesium chelatase subunit H - chloroplast precursor; Chlamydomonas mutants with defects in this protein are chl1 and brs-1 and result in a brown phenotype [PMID: 11713666; PMID: 4436384]. Orthologous to the bacterial protein BchH [PMID: 9359397]; binds protoporphyrin and is acted upon by the ChlI:ChlD complex for magnesium insertion [PMID: 11469861]; interacts with GUN4 and may be involved in chloroplast signalling: Gene is also known as GUN5 in Arabidopsis thaliana [PMID: 11172074; 12574634]; transcript is light regulated and may be diurnal and/or circadian regulated [PMID: 16228385].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1928
    Illegal BglII site found at 2234
    Illegal BglII site found at 3620
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 486
    Illegal NgoMIV site found at 2152
    Illegal AgeI site found at 1132
    Illegal AgeI site found at 2650
    Illegal AgeI site found at 2704
    Illegal AgeI site found at 2923
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2254
    Illegal BsaI.rc site found at 3049
    Illegal SapI.rc site found at 2979


[edit]
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Parameters
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