Composite

Part:BBa_K1163102

Designed by: Emiel van der Kouwe   Group: iGEM13_Evry   (2013-09-22)
Revision as of 20:41, 22 September 2013 by Eccho (Talk | contribs)

pAceB-sfGFP-Term

Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive.

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.

Why would you use this part?

This part allows the characterization of the AceB promoter region thanks to the sfGFP downstream.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 319


[edit]
Categories
Parameters
None