Part:BBa_F2620
3OC6HSL -> PoPS Receiver
A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.
Usage and Biology
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., Part:pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as aTc can be used to produce a logical AND function.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1004
Device Characteristics
control | R0040 |
device_type | Receiver |
family | -NA- |
latency | Seconds |
proteins | LuxR, TetR |
signalling_molecule | 3OC6HSL |
switch_point | 2nM |
Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]
Transfer Function
Latency
Specificity
[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]
Stability
[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]
//function/cellsignalling
control | R0040 |
device_type | Receiver |
family | |
latency | Seconds |
proteins | LuxR, TetR |
signalling_molecule | 3OC6HSL |
switch_point | 2nM |