Regulatory

Part:BBa_K1163101:Design

Designed by: Emiel van der Kouwe   Group: iGEM13_Evry   (2013-09-22)
Revision as of 19:38, 22 September 2013 by Registry (Talk | contribs)

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pAceB promoter region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We blindly extracted the 300 bp sequence upstream of the AceB gene in E. coli. It contains a putative FUR binding site and a putative RBS.


Source

Genomic DNA.

References