Composite
LasR-mCher

Part:BBa_K1157014

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)
Revision as of 16:04, 22 September 2013 by Darlisereno (Talk | contribs)

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pLas-LasR-GFPmut3-Pc-LasR

This biosensor is built to sense C-12 AHL which originates from Pseudomonas aeruginosa. It uses GFP as a reporter, and constitutively express Rhl receptor. When binding with AHL molecules, Rhl receptor will regulate promoter sequence(pRhl) and express GFP which can be detected. A positive-feedback design is involved in order to sense low-level AHL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 1810
    Illegal NheI site found at 1833
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 508
    Illegal NgoMIV site found at 2189
    Illegal AgeI site found at 705
    Illegal AgeI site found at 2386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1582


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Categories
Parameters
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